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Antibodies
Anti-HEC1/HEC antibody [9G3]
Anti-HEC1/HEC antibody [9G3] from Abcam
Be the first to write a review!
Citations:
(67)
Supplier Page
Supplier Page from
Abcam for
Anti-HEC1/HEC antibody [9G3]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-HEC1/HEC antibody [9G3]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab3613
Antibody Type
Primary
Quantity
100 UL
Clonality
Monoclonal
Host
Mouse, Nude mouse
Isotype
IgG2a
Clone
9G3
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
Conjugate/Tag
Unconjugated
Concentration
1 mg/ml
Gene Name
NDC80
Target
HEC1
Reactivity
Hu, Pg
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (67)
(1)
Dynamic phosphorylation of CENP-N by CDK1 guides accurate chromosome segregation in mitosis
Journal of molecular cell biology
November 27, 2023
Liu R, Dou Z, Tian T, Gao X, Chen L, Yuan X, Wang C, Hao J, Gui P, Mullen M,
et al
.
Cell Signaling Technology
,
49426
), anti-
Nuf2
(
Abcam
,
ab122962
), ACA (Immunovision, HCT-0100),
anti-HEC1
(
Abcam
,
ab3613
), anti-CENP-L (kindly gifted by Dr Iain Cheeseman), and anti-CENP-N (kindly gifted by Dr Iain Cheeseman)
(2)
A bifunctional kinase–phosphatase module balances mitotic checkpoint strength and kinetochore–microtubule attachment stability
The EMBO Journal
October 16, 2023
Andrea Corno, Marilia H Cordeiro, Lindsey A Allan, Qian‐Wei Lim, Elena Harrington, Richard J Smith, Adrian T Saurin
(
ab200061
from
Abcam
,
1:1,000
),
mouse
anti‐MAD1
(
MABE867
from
Millipore
,
1:1,000
),
mouse
anti‐HEC1
(
ab3613
from
Abcam
,
1:2,000
) and mouse anti‐FLAG(M2) (
F3165
‐.2MG from
Sigma
,
1:2,000
). The rabbit anti‐pMELT‐KNL1
(3)
Oocytes can repair DNA damage during meiosis via a microtubule-dependent recruitment of CIP2A-MDC1-TOPBP1 complex from spindle pole to chromosomes
Nucleic acids research
June 9, 2023
Leem J, Kim JS, Oh JS.
in an
IBMX
-free medium for 4 h and then microinjected with
CENP-A
(
2048S
,
Cell Signaling
) or
HEC1
(
ab3613
,
Abcam
) antibodies at a final concentration of 100 ng/μl. Control
oocytes
were injected with normal
(4)
Coordination between the Ndc80 complex and dynein is essential for microtubule plus-end capture by kinetochores during early mitosis
Journal of Biological Chemistry
June 1, 2023
Mohammed Abdullahel Amin, Manas Chakraborty, Destiny Ariel Wallace, Dileep Varma
CLIP-170 (200 nM). Antibodies The primary monoclonal
mouse
antibodies used were
anti-Hec1
9G3 (
Abcam
,
ab3613
) for immunoflourescence (IF) at
1:400
and
for WB
at
1:1000
; anti-dynein intermediate chain (
DIC
)
clone
(5)
PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size
The Journal of cell biology
May 1, 2023
Sobajima T, Kowalczyk KM, Skylakakis S, Hayward D, Fulcher LJ, Neary C, Batley C, Kurlekar S, Roberts E, Gruneberg U,
et al
.
sc-515550
; for
immunoprecipitation
only: rabbit pAb,
ProteinTech
,
18932-1-AP
and
mouse
mAb,
Abcam
,
ab3613
; for
immunofluorescence
:
mouse
mAb [
clone
9G3],
ab3613
, Abcam),
MPS1
(rabbit pAb,
10381-1-AP
;
ProteinTech
),
(6)
Semi-automated 3D fluorescence speckle analyzer (3D-Speckler) for microscope calibration and nanoscale measurement
Journal of Cell Biology
April 3, 2023
Jonathan Loi, Xiaofei Qu, Aussie Suzuki
Department of Biochemistry, Stanford University, Stanford, CA, USA; Wan et al., 2009),
mouse
anti-Hec1
(9G3,
ab3613
;
Abcam
), and
mouse
anti-tubulin (#3873; DM1A, mAb). Minimum cross-react
Donkey
anti-rabbit and
mouse
(7)
Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly
Nature communications
November 27, 2022
Matković J, Ghosh S, Ćosić M, Eibes S, Barišić M, Pavin N, Tolić IM.
Abcam
, Cambridge, UK), diluted
1:500
, Rabbit anti-Aurora B (
ab239837
,
Abcam
), diluted
1:100
,
anti-Hec1
(
ab3613
,
Abcam
), diluted
1:100
,
anti-Spindly
(A301-354A, Biomolecules), diluted
1:100
,
anti-phospho-CENP-A (Ser7
)
(8)
Micronuclei from misaligned chromosomes that satisfy the spindle assembly checkpoint in cancer cells
Current Biology
October 10, 2022
Ana Margarida Gomes, Bernardo Orr, Marco Novais-Cruz, Filipe De Sousa, Joana Macário-Monteiro, Carolina Lemos, Cristina Ferrás, Helder Maiato
RRID:AB_10693556
mouse
anti-Aim1,
BD Biosciences
,Cat#
611083
; RRID:AB_398396
mouse
anti-Hec1
(9GA),
Abcam
,
Ab3613
; RRID:AB_303949
mouse
anti-Dsn1,Gift from A. Musacchio,N/A
mouse
anti-
ATRX
,
Santa Cruz Biotechnology
,
sc-55584
;
(9)
Phosphorylation of CENP-R by Aurora B regulates kinetochore-microtubule attachment for accurate chromosome segregation
Journal of molecular cell biology
September 27, 2022
Sedzro DM, Yuan X, Mullen M, Ejaz U, Yang T, Liu X, Song X, Tang YC, Pan W, Zou P,
et al
.
Technology), mouse anti-FLAG (
F3165
,
Sigma
), anti-Cyclin B1 (
12231
,
Cell Signaling Technology
),
anti-Hec1
(
AB3613
,
Abcam
), anti-Aurora B (
611082
,
BD
), anti-CENP-U (
HPA022048
,
Atlas
), and
human
anti-
centromere
auto-antibody
(10)
Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubules
Nature Communications
August 10, 2022
Cédric Castrogiovanni, Alessio V. Inchingolo, Jonathan U. Harrison, Damian Dudka, Onur Sen, Nigel J. Burroughs, Andrew D. McAinsh, Patrick Meraldi
anti-α-tubulin (
1:30054
), rabbit anti-α-tubulin (
1:1000
;
Abcam
;
ab18251
),
mouse
anti-Hec1
(
1:700
;
Abcam
;
ab3613
), rabbit anti-Mad2 (
1:1000
;
Bethyl
;
A300-301A
), and mouse anti-EB1 (
1:200
;
BD biosciences
;
610535
).
(11)
The Astrin-SKAP complex reduces friction at the kinetochore-microtubule interface
Current Biology
June 1, 2022
Miquel Rosas-Salvans, Renaldo Sutanto, Pooja Suresh, Sophie Dumont
J. DeLuca25), CREST (
1:100
,
human
,
Antibodies Incorporated
,
15–234
-0001),
Hec1
(
1:200
,
mouse
,
Abcam
,
ab3613
), tubulin (
1:2000
,
rat
,
Bio-rad
,
MCA77G
). There washes in IF buffer (10 min each) were done before
incubation
(12)
Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals
Cell reports
April 5, 2022
Almeida AC, Soares-de-Oliveira J, Drpic D, Cheeseman LP, Damas J, Lewin HA, Larkin DM, Aguiar P, Pereira AJ, Maiato H.
polyclonal anti-
TPX2
,
Proteintech
,"Cat#
11741-1-AP
, RRID:AB_2208895"
Mouse
monoclonal
anti-HEC1
,
Abcam
,"Cat#
ab3613
, RRID:AB_303949" Rabbit polyclonal anti-Mis12,"Gift from C. Sunkel (Feijão et al., 2013)",N/A Rabbit
(13)
Non-centrosomal microtubules at kinetochores promote rapid chromosome biorientation during mitosis in human cells
Current Biology
March 1, 2022
Fioranna Renda, Christopher Miles, Irina Tikhonenko, Rebecca Fisher, Lina Carlini, Tarun M. Kapoor, Alex Mogilner, Alexey Khodjakov
were obtained as previously described26. KTs and MTs were visualized with monoclonal 9G3/Hec1 (
Abcam
ab3613
) at
1:200
and DM1α antibody (
Sigma
T9026
) antibodies followed by isotype-specific secondary antibodies
(14)
Recruitment of two Ndc80 complexes via the CENP-T pathway is sufficient for kinetochore functions
Nature communications
February 14, 2022
Takenoshita Y, Hara M, Fukagawa T.
rabbit anti-
chicken
Aurora B34 (
1:5000
),
mouse
anti-H3T3ph34 (
1:10000
),
mouse
anti-
human
Ndc80 (
Abcam
,
ab3613
) (
1:1000
), mouse anti-FLAG-M2 (
Sigma
,
F1804
) (
1:1000
),
mouse
anti-α-tubulin (Sigma
T9026
) (
1:5000
),
(15)
Subcellular Euclidean distance measurements with multicolor fluorescence localization imaging in cultured cells
STAR Protocols
December 1, 2021
Tsvetelina E. Germanova, Emanuele Roscioli, Jonathan U. Harrison, Andrew D. McAinsh, Nigel J. Burroughs
monoclonal anti-
CenpA
(
1:300
),
Enzo
,Cat#
ADI-KAM-CC006-E
"
Mouse
monoclonal
anti-Hec1
(9G3,
1:1000
)",
Abcam
,Cat#
ab3613
Mouse
monoclonal anti-Bub1 (
1:200
),
Abcam
,Cat#
ab54893
Mouse
monoclonal anti-Rod (
1:50
),
Abcam
,Cat#
ab56745
Mouse
(16)
Chromosome oscillation promotes Aurora A-dependent Hec1 phosphorylation and mitotic fidelity
The Journal of cell biology
July 5, 2021
Iemura K, Natsume T, Maehara K, Kanemaki MT, Tanaka K.
methods Plasmids and antibodies Monoclonal
mouse
antibodies were used as follows:
anti-Hec1
9G3 (
Abcam
;
ab3613
)
for Western blotting
(WB)
1:2,000
, for IF
1:1,000
; anti–α-tubulin B-5-1-2 (
Merck
;
T5168
) WB
1:5,000
,
(17)
VTT-006, an anti-mitotic compound, binds to the Ndc80 complex and suppresses cancer cell growth in vitro
Oncoscience
January 1, 2021
Laine LJ, Mäki-Jouppila JHE, Kutvonen E, Tiikkainen P, Nyholm TKM, Tien JF, Umbreit NT, Härmä V, Kallio L, Davis TN,
et al
.
phospho-Thr232 (
Rockland
600-401-677
), Bub1 (Upstate, 05-899), BuBR1 (
Abcam
,
ab4637
), Hec1 (
Abcam
,
ab3613
), pericentrin (
Abcam
,
ab4448
), tubulin (
Abcam
,
ab7291
) or
human
autoimmune serum (Crest, Antibodies
(18)
H3K9me3 maintenance on a Human Artificial Chromosome is required for segregation but not centromere epigenetic memory
Journal of Cell Science
July 15, 2020
Nuno M. C. Martins et al.
anti-Aurora B,
1:1000
dilution (
ab2254
,
Abcam
, Cambridge, MA); rabbit anti-Hec1,
1:10,000
dilution (
Ab3613
,
Abcam
), rabbit anti-
Gallus gallus
CENP-A,
1:500
dilution (Régnier et al., 2003);
human
, ACA
1:100
dilution
(19)
Effects of malleable kinetochore morphology on measurements of intrakinetochore tension
Open biology
July 1, 2020
Renda F, Magidson V, Tikhonenko I, Fisher R, Miles C, Mogilner A, Khodjakov A.
Thermo Fisher Scientific
). Outer
kinetochore
domain was stained with a monoclonal 9G3/
Hec1 antibody
(
Abcam
ab3613
) at
1 : 1000
or a rabbit polyclonal antibody against a C-terminal of
CenpF
(
NB500–101
;
Novus
Biological
(20)
Mitotic checkpoint protein Mad1 is required for early Nup153 recruitment to chromatin and nuclear envelope integrity
Journal of Cell Science
May 21, 2020
Ikram Mossaid, Guillaume Chatel, Valérie Martinelli, Marcela Vaz, Birthe Fahrenkrog
(Abcam, Cambridge, UK; ab40567; IF
1:60
); mouse anti-FLAG (
Sigma-Aldrich
;
F3165
; WB
1:2000
);
mouse
anti-Hec1
(
Abcam
;
ab3613
; IF
1:200
);
mouse
mAb414 (Covance, Mechelen, Belgium; IF
1:5000
);
mouse
anti-importin β (
BD Biosciences
,
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Anti-HEC1/HEC antibody [9G3] from Abcam
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